Extended Data Fig. 1: Development and characterization of Kacme antisera.
From: Acetyl-methyllysine marks chromatin at active transcription start sites
a, Chemical structures of lysine post-translational modifications referenced in this work. b, Scheme for synthesis of Fmoc-Lys(Ac,Me)-OH for peptide synthesis. i, CuCO3·Cu(OH)2, Ac2O; ii, 8-hydroxyquinoline; iii, NaHCO3, Fmoc-OSu. c, Chemical structure of the peptide library used to generate Kacme antisera. Yellow box indicates the Kacme residue. d, Peptide dot blots of Kacme antisera with synthetic histone peptides. Yellow boxes indicate Kacme peptides. e, Coomassie stain of Kacme antisera after Protein G purification and SDS-PAGE. Gels were repeated independently twice with similar results. f, Peptide dot blots of Kacme antisera with synthetic peptides. Yellow boxes indicate Kacme peptides. g, Peptide dot blots of purified Kacme antisera with synthetic peptides. Yellow boxes indicate Kacme peptides. h, Western blot of recombinant histone H4 and extracted histones from HEK293T cells with Kacme antisera. i, Western blots of histones extracted from indicated cell types using Kacme antisera pre-incubated with indicated competitor peptide library. Dm, Drosophila melanogaster; Hs, Homo sapiens; Mm, Mus musculus. h-i, Blots were repeated independently twice with similar results.
