Extended Data Fig. 7: Potential reverse correlation between the level of initial NMDAR blockade and the level of recovery.
From: Sustained antidepressant effect of ketamine through NMDAR trapping in the LHb
a, NMDAR-eEPSCs (normalized by baseline) during incubation and wash-out of ketamine (10 μM or 100 μM). NMDAR-eEPSCs are isolated by application of PTX and NBQX in Mg2+ free ACSF under voltage clamp at −70 mV. Bin is 1 min. Each line represents one recorded cell. The red line shows an example cell with no recovery and the green line shows an example cell with 29 % recovery. The black line is the average of all recorded cells. b, Illustration of the calculation of maximal blockade, blockade at 50–60 min and recovery percentage. The maximal blockade is averaged by the 10-min values during maximal blockade period (there is some variation among individuals, but mostly between 0 min to 20 min). Recovery percentage is calculated as: maximal blockade – blockade at 50–60 min. c, Bar graph showing the maximal blockade and blockade at 50–60 min of each cell after ketamine perfusion. Each color represents one recorded cell. d, Recovery percentage plots against maximal blockade of LHb-NMDAR-eEPSCs. Each color represents one recorded cell. (see Supplementary Table 1 for statistical analyses and n numbers).
