Extended Data Fig. 9: 40 Hz LH-LHb stimulation induces LHb burst firing and kicks off trapped ketamine in vivo.
From: Sustained antidepressant effect of ketamine through NMDAR trapping in the LHb
a, Schematic of viral construct, viral injection in the LH of CRS mice. b, Experimental paradigm. c, Schematic of whole-cell recording of LHb neurons in vitro and representative trace showing burst firing elicited by pulsed light stimulation (635 nm, 40 Hz, 2 ms pulse, 0.5 mW) of LH terminals in LHb brain slices. d, Experimental paradigm for electrophysiological recording immediately after optical stimulation in CRS mice. Optical stimulation (635 nm, 40 Hz, 2 ms pulse, 3 min) of LH-LHb terminals or no stimulation is delivered at 1 h after ketamine injection. e-g, NMDAR-eEPSCs (e), AMPAR-eEPSCs (f) and NMDA/AMPA ratio (g) of LHb neurons recorded immediately after optical stimulation from experiment in (d). * P < 0.05, NS, not significant. Error bars indicate SEMs. (see Supplementary Table 1 for statistical analyses and n numbers).
