Fig. 4: Neural activity untraps ketamine from NMDARs.
From: Sustained antidepressant effect of ketamine through NMDAR trapping in the LHb
a, Top left, schematic of a kick-off protocol used to untrap ketamine, including five 3-s pairings of presynaptic stimulation (1 Hz) with postsynaptic depolarization (to +10 mV) delivered over a 5-min period. Bottom left, NMDAR-eEPSCs (normalized by baseline) during incubation and washout of ketamine (10 μM) under three conditions: no stimulus, no extra stimulation; kick-off, two blocks of kick-off activity given after ketamine washout; pre alone, two blocks of presynaptic stimulation alone without postsynaptic depolarization given after ketamine washout. Top right, input resistance of recorded neurons during baseline, wash-in (at 10 min) and washout (at 50 min) periods for three different conditions. b, Bar graphs showing NMDAR-eEPSCs at 50–60 min. Error bars indicate the s.e.m. (see Supplementary Table 1 for statistical analyses and n numbers).
