Extended Data Fig. 2: Single injection of ketamine causes prolonged suppression of LHb bursting activity.
From: Sustained antidepressant effect of ketamine through NMDAR trapping in the LHb
a, Pie charts illustrating the percent abundance of the three types of LHb neurons in naïve mice. b, Pie charts illustrating the percent abundance of the three types of LHb neurons in CRS mice 3 d after saline or ketamine i.p. injection. c, d, Bar graphs illustrating the bursting spike frequency (number of bursting spikes per second,c) and bursts per min (number of bursts per minute, d) in CRS mice at different time points after saline or ketamine i.p. injection. e, Percentage of blockade of bursting spike frequency or bursts per min at each time point calculated as (saline value - ketamine value)/saline value. f, Recording sites of electrodes in LHb. Black lines indicate location of habenula, green dots indicate recording sites of saline group, blue dots indicate recording sites of ketamine group. g, Example traces showing in vivo neuronal activity recorded in CRS mice 3 d after saline or ketamine administration. Bursts (pink shades) are identified by the ISI method (see Methods). h, i, Bar graphs illustrating the bursting spike frequency (h) and bursts per minute (i) in CRS mice 3 d after saline or ketamine i.p. injection. j, k, Percentage of blockade of bursting spike frequency (j) and bursts per minute (k) at each time point calculated as (baseline value - value of each time point)/baseline value. * P < 0.05; ** P < 0.01; *** P < 0.001; NS, not significant. Error bars indicate SEMs. (see Supplementary Table 1 for statistical analyses and n numbers). This figure is related to Fig. 1.
