Extended Data Fig. 4: Neurons targeted in genetic driver lines (supplement to Fig. 4).
From: Flexible circuit mechanisms for context-dependent song sequencing
a, Male brain expressing CsChrimson.mVenus via GMR42B01 ∩ Dsx (green). Neuropil is labeled with nc82 (magenta). The intersection labels pC2 neurons (circled in blue), as well as 6 pCd-like neurons (circled in yellow). These pCd-like neurons do not express Fru (see panel c), and therefore constitute a different subset of neurons than the pCd neurons contributing to persistent male arousal downstream of P1a neurons in28. Broad-range optogenetic activation in males using the genetic driver for pCd neurons from28 produces no song (data not shown). b, Zoom of the boxed area in a), showing that the pC2 population labeled in the intersection consists of both pC2l neurons (solid circle) and pC2m neurons (dashed circle). c, Male brain expressing CsChrimson.mVenus via GMR42B01 ∩ Fru (green). Neuropil is labeled with nc82 (magenta). d, Male brain and VNC expressing CsChrimson.mVenus (green) via both P1a and pIP10 drivers. Neuropil is labeled with nc82 (magenta). Cell bodies of P1a are circled in red. Cell bodies of pIP10 are circled in cyan (see Supplementary Table 2 for full genotypes).
