Extended Data Fig. 1: Identification of IgM in blood serum as the Leishmania clumping factor.
From: Leishmania genetic exchange is mediated by IgM natural antibodies
(a) Gel filtration chromatography of inactivated dog plasma on Sephacryl S-200. Buffer: 20 mM sodium phosphate (pH 7.4), 150 mM NaCl, 5 mM EDTA. Bar represents HPLC fractions corresponding to Leishmania clumping activity. (b) SDS-PAGE of fractions obtained from gel filtration chromatography shown in panel A. Bar represents fractions containing clumping activity that were pooled for ion exchange chromatography. (c) Ion exchange chromatography of pooled fractions 11–13 from B. HPLC gradient, a sodium chloride gradient of 0 – 1 M in 20 mM sodium phosphate, pH 6.0. Bar represents fractions pooled for high resolution gel filtration chromatography. (d) High-resolution gel filtration chromatography on Superdex-200. Buffer: 20 mM sodium phosphate (pH 7.4), 150 mM NaCl. Bar represents fractions pooled for protein identification. (e) Mass spectrum analysis of the most abundant proteins in pooled fractions from (d).
