Fig. 2: Specific PAM DANs recapitulate 0273-neuron-mediated reward seeking.
From: Dopaminergic systems create reward seeking despite adverse consequences
a, Representative GFP expression in DANs driven by 0273-GAL4 combined with different GAL80 transgenes: R15A04-GAL80, R48B04-GAL80 or R58E02-GAL80. Mushroom body is co-labelled with RFP for reference. Three brains were examined for 0273-GAL4, four brains were examined for the other genotypes. b, R58E02-GAL80 produces the greatest reduction in number of PAM somata per hemisphere labelled by 0273-GAL4, followed by R48B04-GAL80 then R15A04-GAL80 (left to right: n = 6, 8, 8 and 8). c, Left, schematic and experimental protocol. Right, R48B04-GAL80 produces the greatest shock-induced reversal of reward seeking driven by 0273 neurons, followed by R58E02-GAL80. R15A04-GAL80 has no significant effect (n = 16). d, Left, schematics and experimental protocol. Bottom, starved flies trained with R48B04-GAL4 (with or without R15A04-GAL80) neuron activation do not show the time-dependent increase in CS+/90 V avoidance observed in R15A04-GAL80 controls (n = 12). Different letters above bars in b–d indicate groups that are significantly different from each other (P < 0.05; one-way ANOVA then Tukey’s HSD; comparisons in d only within genotypes). e, Preference for CS+/90 V is similar for flies harbouring memory implanted by activation of 0273 neurons or β′2&γ4 DANs (left to right: n = 11, 10, 10, 10, 10 and 11; protocol as in c). Different letters above bars indicate treatments that are significantly different (P < 0.05; two-way ANOVA then Tukey’s HSD; main effect of treatment: F(2,56) = 262.1, P < 0.0001). Data are mean ± s.e.m.; dots are individual data points that correspond to individual hemispheres (b) or independent behavioural experiments (c–e). Exact statistical values and comparisons are presented in Supplementary Information.
