Fig. 5: Reward DANs receive diverse and heterogeneous input.
From: Dopaminergic systems create reward seeking despite adverse consequences
a, Volumetric reconstructions of β′2 DANs (PAM02, PAM05 and PAM06 (blue)) and γ4 DANs (PAM08 (red)) within the FlyEM hemibrain (dark grey) overlaid on a complete standard fly brain (light grey). b, Frontal view of volumetric reconstructions of the 402 USNs constituting the top 200 most strongly connected clusters to β′2 and γ4 DANs. USNs are rendered in hues of colours according to neurite location and are shown within the hemibrain neuropil (grey). Additional orientations and reconstructions of all 1,718 USNs connected to β′2 and γ4 DANs are presented in Extended Data Fig. 7 and Supplementary Video 1. c, Network diagram of USNs (inner cream circle) to β′2, γ4 and γ5 DANs (outer wedges) reveals a highly parallel input structure (thresholded at 0.4% of dendritic inputs for visibility). Individual DANs in outer wedges (grey circles) are grouped according to their compartments and types (different coloured wedges) and by subtype. USN clusters are grouped by connectivity pattern (dotted outlines) and connectivity to either one DAN type (circles) or multiple DAN types (squares) is denoted. Outlined USN clusters are labelled with corresponding DAN type targets; triangles mark USN groups that also innervate γ4<γ1γ2 DANs. USN node colours match those in b. Connector weight and transparency represents the percentage of dendritic input to these DANs provided by that USN (range 0.4% to 12.17%). See Extended Data Fig. 8 for a non-thresholded connectivity heat map and Supplementary Tables 1 and 2 and Methods for all connectivity information.
