Extended Data Fig. 1: mSwAP-In design and development.
From: Mouse genome rewriting and tailoring of three important disease loci
(a) Alternative marker cassettes compatible with genetic backgrounds harboring preexisting drug resistance genes. PB ITR, piggyBac inverted terminal repeat; UGT, universal gRNA target. (b) mESC kill curve for each mSwAP-In selection marker. Selected concentrations are highlighted in green: 0.8 μg/ml for puromycin, 8 μg /ml for blasticidin, 150 μg/ml for neomycin, 2.5 μM for 6-thioguanine, 250 nM for ganciclovir and 100 μg/ml for hygromycin. (c) Capture-seq analysis of Hprt deletion. Sequencing reads were mapped to mm10. (d) The bystander effect of thymidine kinase can be overcome by plating single colonies. As few as 0.1% TK-negative cells can be isolated. GCV, ganciclovir (250 nM). Scale bar is 1 mm.
