Extended Data Fig. 9: UGDH is elevated in cancer subsets.
From: Disruption of sugar nucleotide clearance is a therapeutic vulnerability of cancer cells
(a) Immunoblots for UGDH and actin from 11 breast cancer patient tissue samples and 8 normal breast tissues; cancer tissues are labeled in red and normal tissues are labeled in blue. Numbers indicate deidentified patient code, and tumors and normals sharing the same number are adjacent samples from the same patient. (b) Quantification of relative, actin normalized UGDH band intensities from panel a (p = 0.023). (c) Expression profiles of UGDH in 10 types of normal and tumor tissues. The plot is obtained from GEPIA255, a web gene expression profiling tool that plots normalized mRNA-seq data from patient tumor tissues and normal tissues obtained from TCGA and GTEx. All tumor types shown have over 2-fold UGDH expression in tumors than normal tissues (q value < 0.01). q values have been determined by two-way ANOVA and adjusted for false discovery rate. The dotted line indicates mean UGDH TPM of liver normal samples, highest among other normal organs. (d) Top dot plot showing viability following UXS1 KO (y axis) and bottom dot plot showing corresponding UGDH protein expression level (y axis) for 19 cancer and 9 normal cell lines of varying lineage (x axis). Cancer lines are divided between High (red) and Low(gray) UGDH expressing lines based on arbitrary cut-off 1.25 units; normal lines are labeled as blue dots. (e) Immunoblots of UGDH and actin for cancer cell lines and normal cells (non-transformed primary or immortalized cells) shown in Fig. 4h and panel d; UXS1 KO sensitive cell lines are labeled in red and insensitive lines are labeled in gray and normal lines are labeled in blue. (f) Immunoblots for UGDH and actin from 6 normal liver, 6 normal kidney and, 5 normal colon patient tissue samples compared with 2 representative (UGDH high) lung cancer patient samples; cancer tissues are labeled in red and normal tissues are labeled in blue. Data shown in b is shown as mean ± s.d. b, Two-tailed unpaired Student’s t-test.
