Extended Data Fig. 4: Thick filament density projection and profile.
From: Structure of the native myosin filament in the relaxed cardiac sarcomere
a, The three-dimensional map of the thick filament was projected by summing the intensities of the voxels along the x axis or the y axis. b, Intensity profile along the x axis, reveals the periodicity and spacing of the repeating units in the thick filament, in agreement with data from refrozen Tokuyasu cryosections of relaxed rat cardiac muscle72. c, The Fast Fourier Transform (FFT) of the intensity profile shows the peaks resulting from the repetitive nature of its component. The 430 Å peak is associated to the C-type super-repeats while the 143.3 Å peak is associated to the crowns’ layers and was used to calibrate the pixel size of the reconstruction. d, Tomographic volume of a cardiac sarcomere A-band showing thick and thin filaments back-plotted into the reconstructed volume with a single tomographic slice at the center for orientation.
