Extended Data Fig. 10: Myosin OFF state is stabilized by myosin light chains at titin C-type super-repeat 9.
From: Structure of the native myosin filament in the relaxed cardiac sarcomere
a, An overview of the thick filament from the M-band to cMyBP-C stripe No. 4 is provided for context. b, Simplified overview of a single asymmetrical unit at CSR9, showing the molecular arrangement of the myosin crowns, titin and cMyBP-C. In contrast to all other cMyBP-C, the crown 1 does not interact with cMyBP-C CTD at stripe No. 2, instead its FH binds to the RLC from crown 3 and its BH interacts with titin A137. Crown 2 orientation is also atypical as its FH interacts with the disordered region of the ELC of crown 1 and its BH binds to titin A134 (c). Similarly, the FH of crown 3 (A9) binds to the ELC of crown 2 and it is also stabilized by an interaction of its RLC with titin-β A130.
