Extended Data Fig. 2: AmB in one state binds to multiple sterols in AmB-sterol complexes.
From: Tuning sterol extraction kinetics yields a renal-sparing polyene antifungal
a, UV-vis spectra of AmB (black) and AmB upon titration with increasing molar ratios of Erg (0.5, yellow, 1, green, 2, cyan, and 3, blue). The red shift and “finger”-like pattern at higher sterol ratios is consistent with the AmB polyenes separating to accommodate sterols. b, AmB:sterol stoichiometric ratios (average of three replicates) after three rounds of complex washing as described in Methods and a picture of AmB-sterol complexes undergoing sterol washing in chloroform. c, 13C-13C 2D 50 ms DARR spectrum of 13C-AmB:Chol showing AmB in one state. d, 13C-13C 2D SPC5 spectrum with one bond correlations in green and two-bond correlations in blue for a 13C-AmB:13C-Erg sample with Erg crosspeaks highlighted. The largest chemical shift difference observed for each Erg carbon is summarized graphically (SI Table 7). UV-Vis sterol binding interaction between AmB and sterols from ergosterol biosynthetic pathway e, lanosterol, f, zymosterol, and g, episterol.
