Extended Data Fig. 8: Pathway analysis from proteomic data, impact of conditioned medium on organoid growth.

a, b, c, Pathway network analysis highlighting the enrichment/loss of pathways using differentially expressed proteins (DEP) from proteomic data in iMac versus iMicro, NPC versus co-NPC and neuron versus co-neuron, respectively; and showing an increased neurogenesis upon the addition of iMicro. Enrichment is coded with a colour gradient. Prevalence of the pathway is reflected by its p-Value (only pathway with p-Value < 0.05 are represented). d, Images of organoids cultured in the absence (organoid day 0 and day 28) or presence (co-culture organoid) of iMac, or under supernatant transfer from iMac culture for 28 days. Scale bar, 500 µm. n = 6 for each condition. e, Change in the size of brain organoids grown in medium replaced with fresh organoid medium (n = 9) or fresh organoid conditioned medium (n = 9) every 3 days (grey arrows). Statistical analysis, 2-way ANOVA. f, Changes in organoid size generated from XCL-1 iPSC clone over-time in the absence and presence of iMac conditioned medium replaced every 2 days (grey arrows). Co-culture organoids were used as control. Statistical analysis, 2-way ANOVA. (n = 3 per conditions, representative of at least 2 independent experiments). e, f, Error bars, mean +/− s.d. regu. Regulation, pos. positive, neg. negative.