Extended Data Fig. 5: Antigen presentation by CX3CR1 + APCs promote TH1 immunity.
From: Epithelial IFNγ signalling and compartmentalized antigen presentation orchestrate gut immunity
H2abfl/fl and H2abΔiCX3CR1 mice were infected with Citrobacter, injected with tamoxifen on days 3–6 and sacrificed on day 12 p.i. (A) Flow cytometric analyses of MHCII expression in CD172a+ cells from the MLNs of H2abfl/fl and H2abΔiCX3CR1 mice (n = 9 per group). (B) enumeration of GM-CSF- IL-17A+ CD4+ IE-T cells from the MLNs of H2abfl/fl»IfngrΔiIEC and H2abΔiCX3CR1»IfngrΔiIEC chimeric mice after treatments described in Fig. 3g at day 12 p.i. (n = 7 per group). (C) H2abfl/fl and H2abΔiCX3RC1 mice were treated with tamoxifen for 4 consecutive days, followed by infection with Citroova 3 days after the last injection. Mice were injected with CTV-labelled 1 × 106 Rag-OTII cells on day 5 p.i., treated with 20ug of FTY720 i.p. on days 6-8 and sacrificed on day 9 (n = 6 per group). (C) Experimental scheme (D) OTII cells from MLNs were analyzed for T-bet induction. Data were analyzed by (A and D) by Mann-Whitney U test (two-sided) or (B) Kruskal-Wallis test followed by Dunn’s post-test.
