Extended Data Fig. 10: Antigen presentation by IECs restrains pathogenic CD4 T cells that promote chemical colitis.

(A-I) Ifngr1^fl/fl and Ifngr1^fl/flVil^Cre were treated with 1% DSS for 9 days followed by regular drinking water and sacrificed on day 12. (A) Expression of MHCI and (B) MHCII on the epithelial cell surface (n = 6 per group). Expression of CD39 on (C) CD8αβ⁺ and (D) CD4⁺ IE-T cells (n = 5 per group). (E) Quantification of ATP in the colon explants (n = 8 per group) and (F) production of GM-CSF from the CD4⁺ IE-T cells at day 12 (n = 5 per group). (G) Enumeration of neutrophils in the epithelium and (H) Bodyweight change and (I) colon length (n = 6 per group) and representative picture at day 12. (J-M) B2m^fl/fl and B2m^fl/flVil^CreERT2 mice (n = 5 per group) were treated with 1% DSS for 9 days followed by regular drinking water and sacrificed on day 12. Mice were also treated with tamoxifen on days 3-6. (N) Colon lengths from Ifngr1^fl/fl and Ifngr1^fl/flVil^Cre mice treated isotype (n = 10), α-CD4 (n = 8), α-GM-CSF (n = 8), αβ-ATP (n = 10), apyrase (n = 10), MCC950 (n = 10) or α-IL1R (n = 10) during DSS administration and sacrificed on day 12. Each symbol represents an individual mouse, and the bar represents the median except for (H) where each dot indicated the mean and bar indicates the SEM. Data were analyzed by (A-G, I-M) Mann-Whitney U test (two-sided) or (H) ANOVA followed by Sidak post-test or (N) Kruskal-Wallis test followed by Dunn’s post-test.

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