Extended Data Fig. 2: Epithelial sensing of IFN-γ orchestrates T cell responses during colitis.
From: Epithelial IFNγ signalling and compartmentalized antigen presentation orchestrate gut immunity
(A-I) Ifngr1fl/fl and Ifngr1fl/fl VilCre mice were infected with Citrobacter rodentium (n = 5 per group). (A-B) Enumeration of I.E. CD4+ T cells and (C-G) evaluation of production of indicated factors by them at indicated days post-infection. Enumeration of (H-L) indicated cells in the epithelium. (M) Comparison of GM-CSF production from ILCs and CD4+ T cells (n = 7 per group) and (N) GM-CSF MFI in ILCs and CD4+ T cells in epithelium of Ifngr1fl/fl VilCre mice at day 12 p.i. (n = 7 per group). (O) The indicated factors were gated globally on the live cell gate and then analyzed for the contribution of CD8αβ+ or CD4+ colonic IE-T cells in their production. Each symbol represents an individual mouse, and the bar indicates the median. Data were analyzed by (A-L) ANOVA followed by the Holm–Sidak post-hoc test or (M-N) Mann-Whitney U test (two-sided). MFI, median fluorescence intensity.
