Extended Data Fig. 7: Comparison of the Heteronuclear Metal Ion Core.
From: Structural insights into intron catalysis and dynamics during splicing
a. The catalytic elements including the 5′ splice site, branchpoint A, J2/3 linker, catalytic triad, two-nt bulge and the metal ions (M1, M2, K1 and K2 equivalents) for the pre-1F RNP are shown. Density from the cryoEM map of the corresponding regions is shown to indicate model fit. Catalytic core of the b. O.i. intron (PDB: 4FAQ) and c. the spliceosome C complex (PDB:7B9V) and the key mechanistic elements as in (a) are displayed. The attacking water nucleophile is shown as a light blue sphere in (b).
