Extended Data Fig. 4: CryoEM Workflow for the Post-2F RNP.
From: Structural insights into intron catalysis and dynamics during splicing
a. CryoEM data processing workflow for the post-2F RNP (details in methods). b. Local resolution and particle distribution of the post-2F overall and the locally refined post-2F (left) and post-2F (right) maps. c. FSC curves with the gold standard threshold of 0.143 for the three post-2F RNP maps. d. Map to model FSC curve for the post-2F model refined against the post-2F overall map. e. 3DFSC analysis of the post-2F overall, the focused refined post-2F (left) and the focused refined post-2F (right) reconstructions.
