Extended Data Fig. 6: Individual PRLH neurons are activated by food tastes.

a, GRIN lens placement and GcaMP6s expression in PRLH neurons. b, PSTH of PRLH neuron responses during the first lick bout of the trial for the indicated solutions. c, Left, PTSH of activated PRLH neurons (mean z-score > 1 across all 5 s bouts) during Ensure consumption from Fig. 3. Right, PTSH of non-responsive PRLH neurons (mean z-score <1 across all 5 s bouts). d, Percentage of activated and non-responsive neurons during each Ensure lick bout. e, Mean z-score for each 5 s bout during Ensure consumption. Each data point is the averaged value from a single animal. f, Left, heatmap of individual neuron responses to the first bout of Intralipid consumption. Right, heatmap of individual neuron responses to brief access (5 s) Intralipid consumption at 5 min intervals over 20 min. Bottom, PTSH aligned to brief access Intralipid consumption (averaged across all neurons). g, Left, PTSH of activated PRLH neurons (mean z-score > 1 across all 5 s bouts) during Intralipid consumption from Fig. 3. Right, PTSH of non-responsive PRLH neurons (mean z-score <1 across all 5 s bouts). h, Percentage of activated and non-responsive neurons during each Intralipid lick bout. i, Mean z-score for each 5 s bout during Intralipid consumption. j, Left, heatmap of individual neuron responses to the first bout of sucralose consumption. Right, heatmap of individual neuron responses to brief access (5 s) sucralose consumption. Bottom, PTSH aligned to brief access sucralose consumption (averaged across all neurons). k, Left, PTSH of activated PRLH neurons (mean z-score > 1 across all 5 s bouts) during sucralose consumption from Fig. 3. Right, PTSH of non-responsive PRLH neurons (mean z-score <1 across all 5 s bouts). l, Percentage of activated and non-responsive neurons during each sucralose lick bout. m, Mean z-score for each 5 s bout during sucralose consumption. n, Left, heatmap of individual neuron responses to the first bout of water consumption. Right, heatmap of individual neuron responses to brief access (5 s) water consumption. Bottom, PTSH aligned to brief access water consumption (averaged across all neurons). o, Left, PTSH of activated PRLH neurons (mean z-score > 1 across all 5 s bouts) during water consumption from Fig. 3. Right, PTSH of non-responsive PRLH neurons (mean z-score <1 across all 5 s bouts). p, Percentage of activated and non-responsive neurons during each water lick bout. q, Mean z-score for each 5 s bout during water consumption. r, Left, percentage of neurons activated by all four bouts, three bouts, two bouts, or only one lick bout for Ensure consumption. Right, percentage of neurons activated by all four bouts, three bouts, two bouts, or only one lick bout for Intralipid consumption. s, Heatmap of individual neuron responses to brief access sucralose consumption before an IP injection of CCK. NS, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are mean ± sem. Statistics are shown in Supplementary Table 1.