Fig. 3: PRLH neurons are activated by the taste of food.
From: Sequential appetite suppression by oral and visceral feedback to the brainstem
a, Taste impairments in taste-blind Trpm5−/− mice. b, Left, PRLH neuron responses aligned to i.p. injection of CCK in Trpm5−/− mice and WT controls. Right, z scores (0–30 min). c, Left, PRLH neuron responses across all glucose lick bouts in WT and Trpm5−/− mice. Right, z scores (0–10 s). d, Left, PRLH neuron responses across all sucralose lick bouts in WT and Trpm5−/− mice. Right, z scores (0–10 s). e, Top, schematic of microendoscopy imaging of PRLH neurons in a freely moving, head-fixed or head-fixed and restrained mouse. Bottom, example trace of movement artefacts detected using Mosaic analysis software in each configuration during imaging. f, Heatmap of individual neuron responses to the first bout of brief access (5 s) of Ensure consumption. g, Percentage of neurons activated by all four bouts, only three bouts, only two bouts or only-lick bout during Ensure consumption. h, PRLH neuron responses aligned to brief access consumption of Intralipid, sucralose and water (averaged across all neurons). i, PRLH neuron responses aligned to the first lick of all lick bouts (averaged across all lick bouts and neurons). j, Population-weighted z score (calculated as the fraction of neurons activated multiplied by their z-scored activity change) for consumption of the indicated solutions. The percentage of neurons activated are listed above each bar. Statistical comparisons are relative to consumption of water. k, Example traces of calcium dynamics in representative neurons during consumption of the indicated solutions (colours per j). l, Example traces of calcium dynamics in representative neurons responding to sucralose consumption, a CCK injection, both stimuli or neither. m, Left, scatterplot of z scores during brief access sucralose consumption (averaged across all lick bouts) versus z scores after CCK injection. Right, Venn diagram showing the percentage of cells activated by these stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are the mean ± s.e.m. Statistics are shown in Supplementary Table 1.
