Extended Data Fig. 5: Conformational adaptations of eEF2 and contact areas with the tRNA2-mRNA translocating module.
From: mRNA reading frame maintenance during eukaryotic ribosome translocation
a, Alignment on 25 S (left) and global eEF2 alignment (right) on eEF2 in TI-5 for GMPPCP bound intermediates TI-1 and TI-3, and GDP-containing intermediates TI-2, TI-4 and TI-5. Atoms are colored on the basis of root-mean-square deviation values of atomic positions relative to TI-5. b, Buried surface measurements corresponding to contacts of the tRNA2-mRNA module with 80 S ribosome (pink) and domain IV of eEF2 (red) in the course of elongation from PRE-H1 to TI-5. c, Unfiltered, unsharpened density of S. cerevisiae eEF2 (e.g. TI-4) contoured at σ = 3.
