Extended Data Fig. 6: mRNA path on the small subunit of the eukaryotic 80 S ribosome.
From: mRNA reading frame maintenance during eukaryotic ribosome translocation
a, Lateral view of the full 40 S small subunit (SSU) of the eukaryotic 80 S ribosome trapped in the PRE-hybrid-1 reconstruction (PRE-H1) reveals the path of messenger RNA (magenta) at the SSU head-body domain interface. Unsharpened, unfiltered high-resolution cryo-EM maps are shown at σ = 3.5. 18 S ribosomal RNA and SSU proteins are colored in light and dark blue, respectively. b, Zoom on the thirteen nucleotide-long messenger RNA and interacting residues of 18 S rRNA and the conserved SSU protein uS12. +1 indicates the first nucleotide of the start codon. c, Close-up view of the interactions between messenger RNA and ribosomal SSU elements in the PRE-H1 complex. π-π stacking is found between −1 and G1150, while the p-π effect is observed in the following interacting pairs: −3 and G904, and +3 and C1637.
