Extended Data Fig. 6: Compared to non-inhibition mice, stimulus-inhibition mice exhibit similar stimulus response orthogonalization but higher response magnitudes during control trials.

a, Response similarity shown separately for non-inhibition mice (n = 5 mice) and stimulus-inhibition mice (n = 3 mice; using no-inhibition control trials only). b, Change in response similarity (same as a but after subtracting the mean response similarity in first 3 trials) shown separately for non-inhibition mice (n = 5 mice) and stimulus-inhibition mice (n = 3 mice). c, Heatmap of change in response similarity shown separately for non-inhibition mice (n = 5 mice) and stimulus-inhibition mice (n = 3 mice) for all sessions. d, Response similarity using neurons located in upper layer 2/3 or lower layer 2/3 (n = 8 mice, two-tailed unpaired t-test between the mean of the first or last 3 datapoints of the two traces, Holm-Bonferroni corrected, first: P = 0.74, last: P = 0.85). e, Mean stimulus-evoked activity per trial (across all S1 and S2 trials) averaged across all stimulus-driven neurons shown separately for non-inhibition mice (n = 5 mice) and stimulus-inhibition mice (n = 3 mice; using control trials only). f, Heatmap of mean stimulus-evoked activity shown separately for non-inhibition mice (n = 5 mice) and stimulus-inhibition mice (n = 3 mice) for all sessions. g, Percent of decrease neurons that showed a similar decrease in response to both S1 and S2 (defined as a similar drop in Ca²⁺ events/second and/or a similar proportional drop in response magnitude to S1 and S2, n = 8 mice). h, Response similarity when using all neurons or when omitting non-differential decrease neurons (n = 8 mice, permutation test between the mean of the first or last 3 datapoints of the two traces, Holm-Bonferroni corrected, first: P = 0.73, last: P = 0.79). Data are mean ± SEM. n.s.: not significant.