Fig. 2: Cytokines induce cell-type-specific transcriptomic responses.
From: Dictionary of immune responses to cytokines at single-cell resolution
a, Heatmaps of the top DEGs per cell type in response to IFNβ, IL-1β and TNF relative to PBS controls. Colour gradient represents log2(FC) (capped at twofold) in comparison with PBS treatment for the respective cell type. b, Number of DEGs (log2(FC) > 0.3 and FDR-adjusted P < 0.05, two-sided Wilcoxon rank-sum test) following each cytokine treatment, grouped by sharing pattern, either specifically overexpressed by one cell type (top) or shared by two or more cell types (bottom). Cell-type combinations with the most shared genes (>16 DEGs in any given treatment) are shown. A maximum of 100 cells per cytokine treatment for each of the 7 representative cell types were sampled to ensure comparability across cell types for this analysis. The x axes span from 0 to the highest DEG counts. c, Upregulated GPs following IFNα1 and IFNβ (top) or IL-1α and IL-1β (bottom) treatment with respect to PBS control. GPs that are significantly upregulated between cytokine and PBS treatment (effect size > 1 and FDR-adjusted P < 0.01, two-sided Wilcoxon rank-sum test) in any cell type are shown. Significant GPs (FDR < 0.05) for each cell type are represented as circles, with the circle size indicating significance and the colour representing the effect size (capped at 10). Representative enriched biological processes (FDR-adjusted P < 0.05; black tiles) for the top-weighted genes in each GP are shown.
