Extended Data Fig. 3: Cell type-specific gene expression changes induced by IFN-α1, IFN-β, IFN-κ, IFN-γ, IL-1α, IL-1β, IL-18, IL-36α, IL-2, IL-4, IL-7, IL-15, IL-3, GM-CSF, and TNF-α.
From: Dictionary of immune responses to cytokines at single-cell resolution
a, Gene expression heatmaps illustrating top genes upregulated by cytokine treatment compared to PBS treatment in any of the cell types shown. All genes shown have at least log2FC > 0.3 and FDR < 0.05 in one or more cell types. Color gradient indicates average log2FC relative to PBS treatment of the same cell type. Genes expressed in fewer than 10% of cells in both cytokine and PBS treatment conditions are denoted as no change. b, Percentage of upregulated DEGs exclusive to a single cell type (green) or shared among two or more cell types (other colors) after cytokine treatment at various DEG cutoff thresholds. Each box shows a different log2FC and FDR threshold for defining DEGs. This is an extension of the analysis in Fig. 2b, showing consistency of the cell type-specific effects irrespective of DEG cutoffs.
