Fig. 2: SPNs from cortical layer 5, red nucleus and cerebellum are transcriptionally and anatomically discrete.
From: A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons
a, UMAP of all SPNs, coloured by types within taxonomy division 1. b, Expression of select genes for neurotransmission machinery. c–e, Expression of select marker genes (top) and representative MERFISH sections (bottom) of corticospinal neurons (c), rubrospinal neurons (d) and cerebellospinal neurons (e). Number labels depicted on the MERFISH panels correspond to SPN types in a and in the global dendrogram in Fig. 1c. f, Confocal microscopy images of retrogradely labelled cervical- (green) and lumbar- (magenta) projecting corticospinal neurons (1), rubrospinal neurons (2) and cerebellospinal neurons (3). Scale, 100 µm. Representative images shown here from n = 3 injected mice. Expression scale in b applies to all expression plots. FN, fastigial nucleus; IP, interposed nucleus; RN, red nucleus; S1FL, primary somatosensory cortex forelimb region; S1HL, primary somatosensory cortex hindlimb region.
