Fig. 3: Principal taxonomic features and expressed functions of the faecal microbiomes of MDCF-2- and RUSF-treated individuals.
From: Bioactive glycans in a microbiome-directed food for children with malnutrition
a, Significant enrichment of taxa (q < 0.1; GSEA) along PC1 of MAG abundance or transcript abundance. NES, normalized enrichment score. b, Carbohydrate-utilization pathways significantly enriched (q < 0.1; GSEA) by treatment group (β1, circles) or the interaction of treatment group and study week (β3, squares). Right, each point represents a MAG transcript assigned to each of the indicated functional pathways (rows), ranked according to the direction and statistical significance of their differential expression in MDCF-2 versus RUSF treated participants (defined as the direction of the fold change × −log10[P]). Transcripts are coloured by their MAGs of origin. The larger, black outlined circles indicate leading-edge transcripts assigned to the pathway described on the left. c, Carbohydrate-utilization pathways significantly enriched (q < 0.1; GSEA) in upper- versus lower-quartile WLZ responders (β1, diamonds), or the interaction between WLZ-response quartile and study week (β3, triangles) (see linear mixed-effects model in the Methods section ‘Microbial RNA-seq analysis of MAG gene expression’). Right, transcripts assigned to each functional pathway. The colouring and outlining of circles have the same meaning as in b. The enrichment of glucuronate and galacturonate pathways was driven by the same transcripts; these pathways were therefore considered to be a single unit. Supporting information is provided in Supplementary Tables 10–14.
