Extended Data Fig. 5: Sequence alignment of the RdRp and Cap domains among nonsegmented negative-sense (NNS) RNA viruses.
From: Structures of the promoter-bound respiratory syncytial virus polymerase
(a) Sequence alignment of the RdRp domain of the L proteins among NNS RNA viruses. The RdRp domain features a conventional right-hand ‘fingers-palm-thumb’ polymerase fold. The ‘fingers’, ‘palm’, and ‘thumb’ regions are colored blue, red, and brown, respectively. The supporting loop and helix were colored orange. The catalytic ‘GDN’ motif is labeled with a magenta star. Highly conserved and identical (100% conservation) residues involved in the interaction with the RNA template are labeled with green and red triangles, respectively. (b) Sequence alignment of the Cap domain of the L proteins among NNS RNA viruses. The catalytic ‘HR’ motif is labeled with a light brown star. The ‘GXXT’ motif is highlighted and colored green. The priming loop region was colored orange with potential priming residues in RSV (W1262), RABV (W1180), and VSV (W1167) highlighted. The secondary structures of the HRSV L identified are shown above the alignment. The alignment graphics were prepared with Multalin and ESPript. The sequences of the L proteins here were obtained from the UniProt website: HRSV, human respiratory syncytial virus (https://www.uniprot.org/uniprotkb/P28887/entry); HMPV, Human metapneumovirus (https://www.uniprot.org/uniprotkb/Q6WB93/entry); SeV, Sendai virus (https://www.uniprot.org/uniprotkb/O55528/entry); MeV, Measles virus (https://www.uniprot.org/uniprotkb/P12576/entry); HeV, Hendra virus (https://www.uniprot.org/uniprotkb/O89344/entry); PIV5, Parainfluenza virus 5 (https://www.uniprot.org/uniprotkb/Q88434/entry); MuV, Mumps virus (https://www.uniprot.org/uniprotkb/P30929/entry); NDV, Newcastle disease virus (https://www.uniprot.org/uniprotkb/Q9DLD3/entry); MARV, Lake Victoria marburgvirus (https://www.uniprot.org/uniprotkb/P31352/entry); ZEBOV, Zaire ebolavirus (https://www.uniprot.org/uniprotkb/Q05318/entry); RABV, Rabies virus (https://www.uniprot.org/uniprotkb/P11213/entry); BEFV, Bovine ephemeral fever virus (https://www.uniprot.org/uniprotkb/Q9E784/entry); VSV, Vesicular stomatitis Indiana virus (https://www.uniprot.org/uniprotkb/P03523/entry).
