Extended Data Fig. 3: Gad1 proteins inhibit Gabija mediated defense.
From: Phages overcome bacterial immunity via diverse anti-defence proteins
(a) Multiple sequence alignment of the original Gad1 from phage phi3T and five Gad1 homologs that were chosen for experimental verification. Conserved residues are in purple. (b) Results of phage infection experiments with eleven phages of the SPbeta group. Data represent plaque-forming units per ml (PFU/ml) of phages infecting control cells (“no system”), cells expressing the Gabija system (“Gabija”), and cells co-expressing the Gabija system and a Gad1 homolog. Shown is the average of three technical replicates, with individual data points overlaid. The SPbeta data presented here are the same as those presented in Fig. 2d.
