Extended Data Fig. 7: Impact and consequence of 7-DHC on phospholipid peroxidation.
From: 7-Dehydrocholesterol is an endogenous suppressor of ferroptosis
a, Rate of initiation (Ri) in each soy PC liposome composition. b, Dynamic light scattering assessment of the impact of different sterols on the integrity of liposomes c, Scheme of the formation of PLPC-OOH, DLPC-OOH and DLPC-2OOH during autoxidation of soy PC that can be analysed by LC-MS/MS using MRM. d, The resulting profiles of PLPC-OOH, DLPC-OOH and DLPC-2OOH formation over time (integrations are relative to an internal standard (prostaglandin B2). e, Calculated rates from linear regression of the data related to d. f, Representative UV-Vis spectra obtained from a sample of soy PC with 8 mol% 7-DHC during autoxidation. Spectra were processed by subtracting the background trace of vehicle liposomes immediately after the addition of DTUN. Loss of 7-DHC was plotted from the 294 nm peak (inset) with concentrations determined from a standard curve from liposomes prepared with soy PC with inhibitor and added 7-DHC (see Supporting Information). g, Standard curve for 7-DHC prepared in either 95% EtOH or in soy PC liposomes with inhibitor. h, Time course of iron/ascorbate mediated oxidation of Egg-PC and sterol consumption in liposomes containing cholesterol, lathosterol or 7-DHC monitored via HPLC-UV detection (235 nm for PCOOH, 205 nm for cholesterol and lathosterol and 275 nm for 7-DHC). i, Quantification of 7-DHC and secondary oxidation products of 7-DHC in HT1080 SC5D/DHCR7 knockout cells expressing empty vector (black) and SC5D (red) upon 200 nM RSL3 with and without 500 nM Lip1 (6 h). Data are the mean ± s.d of n = 6 wells of a 10 cm plate from two independent experiments, *p < 0.05 two-way ANOVA (i). Each reaction (b, d, e, f, h) was repeated three times and is reported as the mean ± s.d for the kinetic plot (d) or error propagation from the slopes of d derived from linear regression.
