Extended Data Fig. 1: Lipidomic characterization of DHCR7-deficient cells.
From: 7-Dehydrocholesterol is an endogenous suppressor of ferroptosis
a, Lipidomics analysis of HT1080 cells expressing a Cas9 containing lentiviral vector co-expressing sgRNA targeting DHCR7 or EGFP as a control. Represented are the total amount of PE containing PUFA and the ratio of mono- to polyunsaturated fatty acids (MUFA/PUFA) in PE species. Data are represented as mean values ± s.d. of n = 3 technical replicates (from 10 cm plate) performed once. b, Fatty acid composition of PE species in the indicated cell lines. Data are representative of mean values ± s.d. of n = 3 technical replicates (6 cm plate) performed twice. c, Principal component analysis of PE composition data.
