Extended Data Fig. 2: DHCR7 deficiency impact on ferroptosis and other cell death modalities.
From: 7-Dehydrocholesterol is an endogenous suppressor of ferroptosis
a and b, Dose-dependent toxicity of RSL3 and FIN56 in DHCR7-Knockout clonal cell lines generated in the HT1080 (a) and PFa1 (b) cell lines. c, Levels of 7-DHC in MDA-MB-435 parental lines untreated and treated with a DHCR7 inhibitor (RB38 [500 nM]) and three independent DHCR7-KO clones. Data are represented as mean values ± s.d. of n = 2 technical replicates (from 10 cm plate) performed once. d, Dose-dependent toxicity of RSL3, FIN56, ML210, Erastin, Atheronal, Brefeldin-A, PLX-4032, Carfilzomib, TBOOH, Auranofin; Bortezomib and Docetaxel in MDA-MB-435 parental cells and the three DHCR7 knockout clones. Cell viability was assessed after 48 h using Alamar blue and data are representative of mean ± s.d. of n = 3 technical replicates of 2 independent experiments.
