Extended Data Fig. 4: Library design for this study.
From: Adding α,α-disubstituted and β-linked monomers to the genetic code of an organism
PylRS libraries used in this work. a, Overview of the seven libraries designed and created. These libraries target a total of 11 amino acid residues in the PylRS active site and employed several types of degenerate codons. NNK codons are depcited as dark red, DBK codons (+lysine codon) as blue, NDT codons as dark green, NRT codons as yellow. For certain sites, custom residue mixes encompassing the most commonly observed mutations were used (1-7 mixes, depcited as grey spheres). All libraries were created with at least 109 independent transformants. N = A, T, G, C; K = G, T; D = G, A, T; B = G, T, C; R = G, A. The custom mixes are described in the methods. b, The eleven amino acid residues targeted for mutagenesis in the PylRS active site are shown in red. Image was rendered using Pymol, based on the PDB structure 2ZIN.
