Extended Data Fig. 4: (Data related Fig. 4): Optogenetic inhibition of the DP-CEA pathway.
From: Top-down control of flight by a non-canonical cortico-amygdala pathway
a, Intersectional approach used for optogenetic terminal inhibition of the DP-to-CeA neuronal projections. The image was adapted from the Allen Brain Atlas (Allen Institute for Brain Science). b, Experimental timeline. c-e, Effect of optogenetic inhibition on centre time (c), centre entries (d), and distance travelled (e) in the OFT (EYFP N = 9 mice, eNpHR N = 9 mice; unpaired t-test, t = 2.357, df = 16; t = 2.813, df = 16; and t = 0.7250, df = 16, respectively). f-h, Effect of optogenetic inhibition on EYFP (N = 9) and eNpHR (N = 9) mice in the high-threat context on f, freezing (LED-on vs LED-off, Mann-Whitney), g, flight (LED-on vs LED-off, Mann-Whitney), and h, speed during WN (Paired t-test t = 3.497, df=8, p = 0.0081). i-k, Effect of optogenetic inhibition in EYFP (N = 9) and eNpHR (N = 9) groups in the low-threat context on i, freezing (LED-on vs LED-off, Mann-Whitney, n.s.), j, flight (LED-on vs LED-off, Mann-Whitney) and k, speed during WN in the eNpHR group (Paired t-test, t = 2.619, df=8, p = 0.307). Data in c-e represented as mean ± s.e.m. Data in f-k represented as mean with individual data points. Two-sided statistical tests were used. **P < 0.01, *P < 0.05.
