Fig. 1: Differentially expressed Lphn3 splice variants couple to different G proteins.
From: Alternative splicing of latrophilin-3 controls synapse formation
a, Schematic of Lphn3 alternative splicing. The asterisks indicate the stop codon. aa, amino acids; HBD, hormone-binding domain; TMR, transmembrane region. b, Genomic organization of the 3′ alternatively spliced exons of the Lphn3 gene. Alternative exons are colour coded on the basis of the percentage spliced in (PSI) in the hippocampus (Extended Data Fig. 1c), with constitutive exons coloured grey. c, Cell-type-specific splicing of Lphn3. Raw data from ribosome-associated transcriptome analyses18 were analysed to calculate the PSI of each exon for excitatory (excit.) and inhibitory (inhibit.) neurons (subtype-specific data are shown in Extended Data Fig. 1c). Statistical analysis for n = 16 biologically independent replicates was performed using two-sided t-tests; P values are shown in the figure. For the box plots, the whiskers extend to the minimum and maximum values, the centre line shows the median value and the box limits show the interquartile range (25th to 75th percentile). d, G-protein coupling and stimulated cAMP levels associated with Lphn3 splice variants. Left, the splice variants. Middle, the G-protein-coupling signal (bioluminescence resonance energy transfer (BRET) signal) from TRUPATH assays. Right, cAMP stimulated by Lphn3 splice variant expression in HEK293 cells. Detailed data are shown in Extended Data Figs. 4 and 5a.
