Extended Data Fig. 3: Diverse patterns of Lphn3 alternative splicing analysed by RT-PCR in different brain regions and at different times of postnatal development.
From: Alternative splicing of latrophilin-3 controls synapse formation
a, Total RNA isolated from the indicated brain regions of C57BL/6 mice at the indicated postnatal developmental timepoints were analysed by RT-PCRs using primers (labelled in arrows) at exon-exon junctions. RT-PCR products were separated by 2% agarose gel electrophoresis; bands are marked based on the predicted sizes of the alternatively spliced variants shown above each gel. Raw gels are in Supplementary Figs. 1 and 2. b, Quantification of the percentage of multiple variants derived from the same PCR primer pairs. Data are presented as mean values ± SEM (n = 3 biological independent animals). Raw gels are in Supplementary Figs. 1 and 2. Note Exon32 of Lphn3 and β-actin has only one product, therefore were not quantified.
