Extended Data Fig. 4: Detailed TRUPATH analyses of G-protein coupling mediated by six different Lphn3 splice variants.
From: Alternative splicing of latrophilin-3 controls synapse formation
a, TRUPATH assays reveal G-protein coupling signals (NET BRET values) mediated by 6 Lphn3 splice variants expressed at different concentrations. HEK293 cells were transfected with Lphn3 expression plasmids at concentrations of 0–300 ng and the TRUPATH signal was monitored as described. Graphs show means ± SEM from independent experiments (n = 3). All signals were normalized to 0 ng transfected baseline. b, Summary graph of the TRUPATH signal at the highest Lphn3 concentration (300 ng). Graphs show means ± SEM from independent experiments (n = 3). All signals were normalized to 0 ng transfected baseline. c, Heatmap plot of the G-protein coupling strength of the indicated Lphn3 variants at the 200 ng and 300 ng Lphn3 plasmid transfection condition.
