Extended Data Fig. 11: heX-embryoid formation from hiPSCs to model human early post-implantation development in vitro.

(a) From an initially mixed state, heX-embryoid cells segregate into WT clusters surrounded by iGATA6 cells. These iGATA6 cells migrate laterally to create a bilaminar boundary on top of the WT clusters. These clusters then undergo lumenogenesis, specification of amnion-like cells, and formation of anterior hypoblast-like and posterior-like pole. Within heX-embryoids, yolk sac mesoderm-like tissue specifies and hematopoietic cell specification is observed. (b) Schematic  comparing heX-embryoid morphology in relation to the human embryo. e The fluorescence image is taken from Extended Data Fig. 1i and is stained for OCT4, GFP, and F-actin. Human E16 embryo image is taken from ref. ²². Illustrations in a and b were created using BioRender (https://biorender.com).