Fig. 3: Anterior hypoblast domain and posterior pole in heX-embryoids.
From: Modelling post-implantation human development to yolk sac blood emergence
a, Merged UMAP of all iGATA6 lineages showing the compartments expressing markers of anterior hypoblast. Two separate domains of these markers were observed, one within day 2 and one within day 3–5 of the embryoid cells. (UMAP labelled by day can be seen in Extended Data Fig. 5). b, Merged UMAP of all WT lineages showing the compartment expressing markers of the posterior pole and primitive streak. c, IF staining showing a z-slice of a heX-embryoid with a HHEX/LHX1/CER1 copositive domain adjacent to a WT cluster. The dotted line indicates the boundaries of the WT cluster in each image, and the arrow indicates the copositive domain. d, IF staining showing a TBXT/MIXL1 copositive domain within the WT clusters of the embryoid. e, Examples of WT clusters with polarized TBXT/MIXL1 domains (top) or without co-expression of markers (bottom). The pie chart shows proportions of cluster types observed in the embryoid cultures, n = 746 total. f, A pie chart showing the proportion of clusters with a particular TBXT/CER1 polarity type when CER1 confined to one pole is present. n = 169 total. g, Representative WT clusters showing TBXT and CER1 expression domains at opposing poles of the cluster (anti-polar, top) or at the same pole of the cluster (syn-polar, bottom). h, Diagrams indicating the average radial expression patterns of TBXT in each polarity pattern from WT clusters with a polarized CER1-expressing domain indicated in f. All diagrams are scaled to the same expression intensity value (1 a.u.). Degrees indicate radial distance around the circularized perimeter of a WT cluster from the CER1 peak shown. Shaded areas indicate region of highest average TBXT polarity corresponding to the polarity types. Scale bars, 100 µm. n represents the embryoid structures from three to four separate experiments harvested on day 4 after induction.
