Extended Data Fig. 5: Immunization does not alter influx of NK cells and monocytes following Sendai virus transmission.
From: Prevention of respiratory virus transmission by resident memory CD8+ T cells
(a) Experimental schematic where immunized contact mice were cohoused with a Sendai-Luc infected index mouse and tissues analyzed for innate immune populations at the time of cohousing (D30) and two days after cohousing (D30 + 2). (b) Number of NK cells in nasal cavity (D30: n = 11 for PR8 WT i.n., n = 15 for PR8-SenNP i.n., n = 14 for PR8-SenNP i.p.) (D30 + 2: n = 11 for PR8 WT i.n., n = 12 for PR8-SenNP i.n., n = 12 for PR8-SenNP i.p.) and BAL (D30: n = 10 for PR8 WT i.n., n = 14 for PR8-SenNP i.n., n = 14 for PR8-SenNP i.p.) (D30 + 2: n = 11 for PR8 WT i.n., n = 11 for PR8-SenNP i.n., n = 12 for PR8-SenNP i.p.). (c) Number of inflammatory monocytes in nasal cavity and BAL (same n as (b), except n = 12 for D30 + 2 PR8-SenNP i.n. in BAL). Data shown are from 3 independent experiments. Lines represent means and error bars represent 95% confidence interval (b, c). Statistical significance was determined using two-sided Mann Whitney test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: non-significant.
