Extended Data Fig. 6: aVMHvlOXTR cells increase response to the aggressor after defeat in female mice.
From: A dedicated hypothalamic oxytocin circuit controls aversive social learning
a. Virus injection site and a representative histology image. Scale bar: 200 µm. Brain illustration is based on a reference atlas from https://atlas.brain-map.org/. b. Experimental timeline and cartoon illustration of the behavior assay. c-e. Summary plots showing the investigation time (c), time around each cup (d), and number of approach (e) during pre- and post-defeat MSI tests. E: Empty; Cv: C57 virgin female; Sv: unfamiliar SW virgin female; Sm: SW lactating female aggressor. f. Representative raw traces showing the Z-scored GCaMP6 signal in the pre- (f1) and post-defeat (f2) MSI tests. Shades represent investigation episodes. Empty cup investigation episodes are not marked. g. PETHs aligned to the investigation onset of different stimuli in pre- and post-defeat MSI tests. h. The mean Z-scored GCaMP6 signal during investigation of different targets in pre- and post-defeat MSI tests. h1 and h2 are the same data shown in different arrangements. i. Scatter plots showing the correlation between change index in investigation time and change in Z-scored GCaMP responses to various social targets after defeat from the pre-defeat level. Plots with error bars and shades represent mean±s.e.m. Circles and lines represent individual animals. Numbers on the plots indicate the number of animals. (c, d, e, h) Two-way repeated measure ANOVA with Sidak’s multiple comparisons test. (i) Pearson cross-correlation. All statistical tests are two-tailed. *p < 0.05, **p < 0.01, and ***p < 0.001. See Supplementary Table 1 for detailed statistics.
