Extended Data Fig. 3: Transient inhibition of epigenetic factors in NPC does not alter cortical patterning and neurogenesis.
From: An epigenetic barrier sets the timing of human neuronal maturation
a, Schematic of experimental paradigm for transient inhibition of chromatin regulators at progenitor cell stage. NPC were treated with small molecule from d12 to d20. b, Small molecule compounds used in the study and corresponding intracellular targets. c, Representative images of d20 NPCs treated with small molecule before the induction of synchronized neurogenesis and stained with antibodies against cortical markers PAX6 and FOXG1, the proliferation marker Ki67 and the neuron marker MAP2. d, Quantification of the fraction of cells expressing PAX6 and FOXG1 at d20 in treated versus control conditions (n = 2 independent experiments). e, Quantification of the fraction of progenitor cells (KI67 +) and neurons (MAP2 +) at d20 in treated versus control conditions. DMSO n = 3, EZH2i n = 3, EHMT1/2i n = 2, DOT1Li n = 3, KDM1Ai n = 3, KDM5i n = 3, KMT5i n = 2 (independent experiments). Histograms depict mean ± s.e.m. Scale bars are 50 μm.
