Extended Data Fig. 1: UBR4 deletion sensitizes cells to mitochondrial protein import stress.
From: Stress response silencing by an E3 ligase mutated in neurodegeneration
a. Western blot analysis shows deletion of endogenous UBR4 from HEK293T cells as a prerequisite for a subsequent whole genome synthetic lethality CRISPR screen. Three different antibodies directed against UBR4 were used to establish successful deletion. b. GO analysis of genetic interactors of UBR4. Screen hits in the top 5% CasTLE score and with negative CasTLE effects were run through ClueGO to identify enriched pathways that are synthetic lethal with UBR4 deletion. P values were generated by ClueGO (Fisher’s exact test and Bonferroni correction). For gel source data, see Supplementary Fig. 1.
