Extended Data Fig. 10: Immunofluorescence of cluster 12 misregulated genes in iNets overexpressing TDP-43-HA.
From: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
a,b Immunofluorescence of iNets transduced to overexpress TDP-43-HA for 4 weeks demonstrating that only the TDP-43-HA-positive neurons accumulated NPTX2 in the soma and neurites c, Quantification of the percentage of TDP-43-HA-expressing neurons with NPTX2 accumulation at 1 and 2 weeks of transgene expression. Unpaired t test. Data shown from a representative experiment (out of n = 2). Shown are mean with SD and p values are two-tailed. 1 W vs 2 W p = 0.0271. Each data point represents a sum of all MAP2+ neurons expressing TDP-43-HA that showed NPTX2 upregulation; expressed as a percentage out of total neuron count per well (182 fields of view). Between 855 - 2722 cells were analyzed per data point. Immunofluorescence of iNets with TDP-43-HA expression for 4 weeks showing increased levels of MEF2A in the nucleus (d) and lowered levels of STMN2 (demonstrated with 2 different antibodies) in the soma and processes (e). MEF2A and STMN2 IF were repeated twice. Scale bars, 20 µm.
