Fig. 5: Clonal structure alterations in human haematopoiesis with ageing.
From: Deciphering cell states and genealogies of human haematopoiesis
a, Comparison of mtDNA mutation burden between young and aged donors across different cell types. n = 11,009, 15,101, 9,519 and 14,715 cells in young-1, young-2, aged-1 and aged-2, respectively (yo, years old). Boxplot shows data from the 25th–75th percentile and whiskers extending to the minimum and maximum within 1.5× IQR. ***P < 2.2 × 10−16, derived from one-sided Wilcoxon rank-sum test. b,c, Phylogenetic trees from young (b) and aged donors (c). Clonal groups are indicated by different colours on the outer rings. d, Contribution of each clone to the total population, in two young and two aged donors. e, Shannon diversity index of clonal composition between young and aged donors. f, Mapping single-cell fitness score and cells with LOY on the phylogenetic tree for aged-1. Raw and smoothed LOY cell distribution is shown in outer rings. P values (and FDR using q-value) of LOY enrichment (one-sided binomial test) are shown. g, Cell-type contribution in each expanded clade. Grey area indicates the expected balanced cell-type distribution.
