Extended Data Fig. 5: Intraperitoneally-injected LIPSTIC substrate reaches cells in multiple tissues.
From: Universal recording of immune cell interactions in vivo
(A) Steady state Rosa26uLIPSTIC/WT.Clec9aCreER/WT or Rosa26uLIPSTIC/WT.Clec9aWT/WT control mice were injected i.p. with the LIPSTIC substrate and its loading onto DCs was achieved in all analyzed tissues. (B-C) I.p. injection of the LIPSTIC substrate reaches the brain in Rosa26uLIPSTIC/WT.Cx3xr1CreERT2/WT mice. (B) Flow cytometry gating strategy to analyze CX3CR1-expressing microglia in the brain. To discriminate resident from circulating immune cells in the brain, α-CD45 antibody was injected intravenously to mark the latter. (C) SrtA expression was detected in ~80% of CD11b+CX3CR1+ cells, 68% of which acquired i.p.-administered LIPSTIC substrate.
