Extended Data Fig. 1: Concept of the redox-based Trp-CLiC strategy.
From: Oxidative cyclization reagents reveal tryptophan cation–π interactions
a) Selectivity challenges posed by traditional probes for tryptophan labelling. Histidine, tyrosine, and cysteine can also be targeted with significantly varying degrees of labelling. b) Reaction scheme of oxidative cyclization reagents for chemoselective tryptophan bioconjugation. The Tryptophan Chemical Ligation by Cyclization (Trp-CLiC) method is highly tryptophan-selective and rapid, and it can generate stable cycloadducts with a diversity of payloads, facilitating antibody bioconjugation, unfolding stress detection, and reactive tryptophan profiling.
