Extended Data Fig. 4: Characterization of the redundant role of PRG-1.1 and PRG-1.2 in C. tropicalis.
From: Selfish conflict underlies RNA-mediated parent-of-origin effects
a, Schematic of the sRNA immunoprecipitation (RIP) protocol. RIP was performed in biological triplicates on N-terminally FLAG-tagged PRG-1.1 and PRG-1.2 strains (tagging the endogenous locus) followed by sRNA-seq. b, Class enrichment among sRNAs bound either to PRG1-1.1 and PRG-1.2 compared to a total sRNA-seq protocol (nPRG-1.1 = 3, nPRG-1.2 = 3). c, Differences in the abundance of piRNAs bounds by each PRG-1 paralog. Normalized abundance (transcript per million) of PRG-1.1 (left) and PRG-1.2 (right) bound piRNAs (21U-RNAs) are plotted on the Y-axis and genomic coordinate of the respective piRNA loci on the X-axis. Differentially bound piRNAs are shown with different thresholds of significance and are enriched within specific genomic clusters (moderated t-test, p-value adjusted with Benjamini & Hochberg correction).
